Podoplanin antibody (C-Term)

Catalog No. ABIN3043111

This Rabbit Polyclonal antibody specifically detects Podoplanin in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Podoplanin antibody (C-Term) (ABIN3043111)

Target: Podoplanin (PDPN)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 155-166, C-Term

Purpose: Anti-Podoplanin/gp36/PDPN Antibody Picoband®

Sequence: VVMRKISGRF SP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Podoplanin/gp36/PDPN Antibody (ABIN3043111). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat Podoplanin/gp36, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Kato, Y., Fujita, N., Kunita, A., Sato, S., Kaneko, M., Osawa, M., Tsuruo, T. Molecular identification of Aggrus/T1-alpha as a platelet aggregation-inducing factor expressed in colorectal tumors. J. Biol. Chem. 278: 51599-51605, 2003. 2. Ma, T., Yang, B., Matthay, M. A., Verkman, A. S. Evidence against a role of mouse, rat, and two cloned human T1-alpha isoforms as a water channel or a regulator of aquaporin-type water channels. Am. J. Resp. Cell Molec. Biol. 19: 143-149, 1998. 3. Schacht, V., Ramirez, M. I., Hong, Y.-K., Hirakawa, S., Feng, D., Harvey, N., Williams, M., Dvorak, A. M., Dvorak, H. F., Oliver, G., Detmar, M. T1-alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J. 22: 3546-3556, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: PDPN

Background:

Synonyms: Podoplanin,E11 antigen epitope,Glycoprotein 38,Gp38,OTS-8,RTI140,T1-alpha,T1A,Type I cell 40 kDa protein,Pdpn,Gp38, Ots8,

Tissue Specificity: In adult kidney, expressed on the urinary surface and foot processes of podocytes and in parietal epithelial cells of Bowman's capsule where it is localized to luminal surfaces. In lung, expressed exclusively on luminal surfaces of type I alveolar epithelial cells and pleural mesothelial cells. Not expressed in type II alveolar cells. In bone, expressed in osteocytes and osteoblasts. In spleen, liver, stomach and intestine, expressed in mesoepithelium. Also expressed in thymic epithelial cells, choroid plexus and leptomeninges. .

Background: PDPN (Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.

Sequence Similarities: Belongs to the heparin-binding growth factors family.

Molecular Weight: 38 kDa

UniProt: Q64294

Pathways: Dicarboxylic Acid Transport