Oncostatin M antibody (Middle Region)

Catalog No. ABIN3043117

Product Details anti-Oncostatin M Antibody

Target: Oncostatin M (OSM)

Binding Specificity: AA 181-198, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Oncostatin M/OSM Antibody Picoband®

Sequence: ASDAFQRKLE GCRFLHGY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Oncostatin M/OSM Antibody (ABIN3043117). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Oncostatin M.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Miles, S. A., Martinez-Maza, O., Rezai, A., Magpantay, L., Kishimoto, T., Nakamura, S., Radka, S. F., Linsley, P. S. Oncostatin M as a potent mitogen for AIDS-Kaposi's sarcoma-derived cells. Science 255: 1432-1434, 1992. 2. Modur, V., Feldhaus, M. J., Weyrich, A. S., Jicha, D. L., Prescott, S. M., Zimmerman, G. A., McIntyre, T. M. Oncostatin M is a proinflammatory mediator: in vivo effects correlate with endothelial cell expression of inflammatory cytokines and adhesion molecules. J. Clin. Invest. 100: 158-168, 1997. 3. Rose, T. M., Bruce, A. G. Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Nat. Acad. Sci. 88: 8641-8645, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Oncostatin M antibody (ABIN3043117)

Zhu, Che, Liao, Wang, Wang, Chen, Wang, Dai, Wan: "Oncostatin M activates STAT3 to promote endometrial cancer invasion and angiogenesis." in: Oncology reports, Vol. 34, Issue 1, pp. 129-38, (2015) (PubMed).

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: OSM (OSM Products)

Background:

Synonyms: Oncostatin-M,OSM,OSM,

Tissue Specificity: Detected in neutrophils (at protein level). .

Background: OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The ion of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.

Sequence Similarities: Contains 1 FAD-binding FR-type domain.

Molecular Weight: 28 kDa

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion