IDO2 antibody (N-Term)

Catalog No. ABIN3043149

Product Details anti-IDO2 Antibody

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Binding Specificity: AA 1-20, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDO2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-INDOL1/IDO2 Antibody Picoband®

Sequence: MLHFHYYDTS NKIMEPHRPN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-INDOL1/IDO2 Antibody (ABIN3043149). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human INDOL1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Ball, H. J., Sanchez-Perez, A., Weiser, S., Austin, C. J. D., Astelbauer, F., Miu, J., McQuillan, J. A., Stocker, R., Jermin, L. S., Hunt, N. H. Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice. Gene 396: 203-213, 2007. 2. Metz, R., DuHadaway, J. B., Kamasani, U., Laury-Kleintop, L., Muller, A. J., Prendergast, G. C. Novel tryptophan catabolic enzyme IDO2 is the preferred biochemical target of the antitumor indoleamine 2,3-dioxygenase inhibitory compound D-1-methyl-tryptophan. Cancer Res. 67: 7082-7087, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IDO2

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Alternative Name: IDO2 (IDO2 Products)

Background:

Synonyms: Indoleamine 2,3-dioxygenase 2,IDO-2,1.13.11.-,Indoleamine 2,3-dioxygenase-like protein 1,Indoleamine-pyrrole 2,3-dioxygenase-like protein 1,IDO2,INDOL1,

Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. .

Background: IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.

Sequence Similarities: Belongs to the indoleamine 2,3-dioxygenase family.

Molecular Weight: 47 kDa