TBP antibody (Middle Region)

Catalog No. ABIN3043164

This anti-TBP antibody is a Rabbit Polyclonal antibody detecting TBP in WB. Suitable for Human, Mouse and Rat.

Quick Overview for TBP antibody (Middle Region) (ABIN3043164)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TBP Antibody

Binding Specificity: AA 227-246, Middle Region

Purpose: Anti-TATA binding protein TBP Antibody Picoband®

Sequence: EEQSRLAARK YARVVQKLGF

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TATA binding protein TBP Antibody (ABIN3043164). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TBP, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Bauer, P., Laccone, F., Rolfs, A., Wullner, U., Bosch, S., Peters, H., Liebscher, S., Scheible, M., Epplen, J. T., Weber, B. H. F., Holinski-Feder, E., Weirich-Schwaiger, H., Morris-Rosendahl, D. J., Andrich, J., Riess, O.Trinucleotide repeat expansion in SCA17/TBP in white patients with Huntington's disease-like phenotype.J. Med. Genet. 41: 230-232, 2004. 2. Stevanin, G., Fujigasaki, H., Lebre, A.-S., Camuzat, A., Jeannequin, C., Dode, C., Takahashi, J., San, C., Bellance, R., Brice, A., Durr, A.Huntington's disease-like phenotype due to trinucleotide repeat expansions in the TBP and JPH3 genes.Brain 126: 1599-1603, 2003. 3. Veenstra, G. J. C., Weeks, D. L., Wolffe, A. P.Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus.Science 290: 2312-2314, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background:

Synonyms: TATA-box-binding protein,TATA sequence-binding protein,TATA-binding factor,TATA-box factor,Transcription initiation factor TFIID TBP subunit,TBP,GTF2D1, TF2D, TFIID,

Tissue Specificity: Widely expressed, with levels highest in the testis and ovary. .

Background: TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20 % of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.

Molecular Weight: 31 kDa

UniProt: P20226

Pathways: WNT Signaling