CD147 antibody (Middle Region)

Catalog No. ABIN3043170

This anti-CD147 antibody is a Rabbit Polyclonal antibody detecting CD147 in WB and IHC. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for CD147 antibody (Middle Region) (ABIN3043170)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD147 Antibody

Binding Specificity: AA 186-203, Middle Region

Purpose: Anti-CD147/BSG Antibody Picoband®

Sequence: QKTEFKVDSD DQWGEYSC

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-/BSG Antibody (ABIN3043170). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CD147.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human1. Guo, H., Majmudar, G., Jensen, T. C., Biswas, C., Toole, B. P., Gordon, M. K.Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases.Gene 220: 99-108, 1998. 2. Zhou, S., Zhou, H., Walian, P. J., Jap, B. K.CD147 is a regulatory subunit of the gamma-secretase complex in Alzheimer's disease amyloid beta-peptide production.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD147 antibody (ABIN3043170)

Zhang, Yang, Liu, Liu, Teng, Zhang, Yin, Liu et al.: "iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of ..." in: Oncology letters, Vol. 14, Issue 3, pp. 3734-3740, (2017) (PubMed).

Xiao, Zhao, Shen, Liang, Chen: "Overexpression of CD147 is associated with poor prognosis, tumor cell migration and ERK signaling pathway activation in hepatocellular carcinoma." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 2637-2642, (2017) (PubMed).

Lu, Lv, Kim, Ha, Kim: "Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma." in: Oncology letters, Vol. 5, Issue 1, pp. 201-207, (2012) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: BSG

Background:

Synonyms: Basigin,5F7,Collagenase stimulatory factor,Extracellular matrix metalloproteinase inducer,EMMPRIN,Leukocyte activation antigen M6,OK blood group antigen,Tumor cell-derived collagenase stimulatory factor,TCSF,CD147,BSG,UNQ6505/PRO21383,

Tissue Specificity: Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Background: Emmprin, extracellular matrix metalloproteinase inducer, also known as Emmprin (BSG) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the Emmprin gene. the BSG gene contains 8 exons and spans 10.8 kb. The human BSG gene is mapped to 19p13.3. This protein is a determinant for the Ok blood group system. Emmprin has been shown to be an essential receptor on red blood cells for the malaria parasite. Emmprin is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, Emmprin is though also to play a role in intercellular recognition. Emmprin also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Emmprin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Sequence Similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Molecular Weight: 35-60 kDa

UniProt: P35613

Pathways: S100 Proteins