PF4 antibody (C-Term)

Catalog No. ABIN3043213

This Rabbit Polyclonal antibody specifically detects PF4 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for PF4 antibody (C-Term) (ABIN3043213)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PF4 Antibody

Binding Specificity: AA 85-101, C-Term

Purpose: Anti-Platelet factor 4 PF4 Antibody Picoband®

Sequence: DLQAPLYKKI IKKLLES

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Platelet factor 4 PF4 Antibody (ABIN3043213). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PF4.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Eisman, R., Surrey, S., Ramachandran, B., Schwartz, E., Poncz, M. Structural and functional comparison of the genes for human platelet factor 4 and PF4-alt. Blood 76: 336-344, 1990. 2. Griffin, C. A., Emanuel, B. S., LaRocco, P., Schwartz, E., Poncz, M. Human platelet factor 4 gene is mapped to 4q12-q21. Cytogenet. Cell Genet. 45: 67-69, 1987. 3. Lambert, M. P., Rauova, L., Bailey, M., Sola-Visner, M. C., Kowalska, M. A., Poncz, M. Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications. Blood 110: 1153-1160, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: PF4

Background:

Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Iroplact,Oncostatin-A,Platelet factor 4, short form,PF4,CXCL4, SCYB4,

Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Background: CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Sequence Similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Molecular Weight: 50 kDa

UniProt: P02776