NAE1 antibody (AA 181-534)

Catalog No. ABIN3043281

This anti-NAE1 antibody is a Rabbit Polyclonal antibody detecting NAE1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for NAE1 antibody (AA 181-534) (ABIN3043281)

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAE1 Antibody

Binding Specificity: AA 181-534

Purpose: Anti-APPBP1/NAE1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-APPBP1/NAE1 Antibody Picoband® (ABIN3043281). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APPBP1 recombinant protein (Position: E181-L534). Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Hong BH, et al. Amyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cells. Neuroreport, 2012 Jan 25. 2. Joo Y, et al. Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells. PLoS One, 2010 Dec 2. 3. Zhong HJ, et al. A metal-based inhibitor of NEDD8-activating enzyme. PLoS One, 2012.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NAE1

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Alternative Name: NAE1

Background:

Synonyms: NEDD8-activating enzyme E1 regulatory subunit,Amyloid beta precursor protein-binding protein 1, 59 kDa,APP-BP1,Amyloid protein-binding protein 1,Proto-oncogene protein 1,NAE1,APPBP1,HPP1,

Tissue Specificity: Ubiquitous in fetal tissues. Expressed throughout the adult brain. .

Background: NAE1 is also known as APPBP1. The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 42 kDa

Gene ID: 8883

UniProt: Q13564