Lipocalin 2 antibody (AA 21-200)

Catalog No. ABIN3043290

This anti-Lipocalin 2 antibody is a Rabbit Polyclonal antibody detecting Lipocalin 2 in WB, ELISA and IHC. Suitable for Rat and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Lipocalin 2 antibody (AA 21-200) (ABIN3043290)

Target: Lipocalin 2 (LCN2)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lipocalin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Lipocalin 2 Antibody

Binding Specificity: AA 21-200

Purpose: Anti-Lipocalin 2/LCN2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Lipocalin 2/LCN2 Antibody (ABIN3043290). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200). Mouse Lipocalin 2 shares 62 % and 81.1 % amino acid (aa) sequence identity with human and rat Lipocalin 2, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
Western blot, 0.1-0.5 μg/mL

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Lipocalin 2 antibody (ABIN3043290)

Narayan, Duan, Jiang, Li, Paka, Yamin, Friedman, Weir, Goldberg: "Late intervention with the small molecule BB3 mitigates postischemic kidney injury." in: American journal of physiology. Renal physiology, Vol. 311, Issue 2, pp. F352-61, (2017) (PubMed).

Li, Wang, He, Chen, Yu: "Expression of neutrophil gelatinase-associated lipocalin in low osmolar contrast-induced nephropathy in rats and the effect of N-acetylcysteine." in: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3175-3180, (2016) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: Lcn2

Background:

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Lipocalin-2,SV-40-induced 24P3 protein,Siderocalin LCN2,p25,Lcn2,

Tissue Specificity: Detected in lung, spleen, uterus, vagina and epididymis. .

Background: Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Sequence Similarities: Belongs to the KIN17 family.

Molecular Weight: 22 kDa

Gene ID: 16819

UniProt: P11672

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis