NFKB2 antibody (AA 1-340)

Catalog No. ABIN3043293

This anti-NFKB2 antibody is a Rabbit Polyclonal antibody detecting NFKB2 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 7+ publications.

Quick Overview for NFKB2 antibody (AA 1-340) (ABIN3043293)

Target: NFKB2 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFKB2 Antibody

Binding Specificity: AA 1-340

Purpose: Anti-NFkB/NFKB2 p100/p52 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® (ABIN3043293). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340). Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. Schmid RM, Perkins ND, Duckett CS, Andrews PC, Nabel GJ (Sep 1991). "Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65". Nature 352 (6337): 733-6. 2. Basak, S., Kim, H., Kearns, J. D., Tergaonkar, V., O'Dea, E., Werner, S. L., Benedict, C. A., Ware, C. F., Ghosh, G., Verma, I. M., Hoffmann, A. A fourth I-kappa-B protein within the NF-kappa-B signaling module. Cell 128: 369-381, 2007. 3. "Entrez Gene: NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)"

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-NFKB2 antibody (ABIN3043293)

Sun, Xue, Xue, Ren, Wu, Wang: "Acetylpuerarin protects against OGD-induced cell injury in BV2 microglia by inhibiting HMGB1 release." in: Die Pharmazie, Vol. 73, Issue 2, pp. 92-97, (2018) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Wang, Wang, Fang, Zhou, Liu, Su: "High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy." in: Diabetology & metabolic syndrome, Vol. 7, pp. 89, (2015) (PubMed).

Yang, Wang, Wang, Xu, He, Wen, Yan, Su, Zhu: "Toll-like receptor 4 prompts human breast cancer cells invasiveness via lipopolysaccharide stimulation and is overexpressed in patients with lymph node metastasis." in: PLoS ONE, Vol. 9, Issue 10, pp. e109980, (2014) (PubMed).

Zhao, Wang, Liu, Sun, Kazuhiro: "Protective effects of glutamine in a rat model of endotoxemia." in: Molecular medicine reports, Vol. 6, Issue 4, pp. 739-44, (2012) (PubMed).

Li, Chen, Zhang, Zhu, Liu, Liang, Sun, Liao, Gong: "Protective effect of glutamine-enriched early enteral nutrition on intestinal mucosal barrier injury after liver transplantation in rats." in: American journal of surgery, Vol. 199, Issue 1, pp. 35-42, (2010) (PubMed).

Target Details for NFKB2

Target: NFKB2 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))

Alternative Name: NFKB2

Background:

Synonyms: Myeloid differentiation primary response protein MyD88,MYD88,

Tissue Specificity: Ubiquitous.

Background: NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.

Sequence Similarities: Contains 7 ANK repeats.

Molecular Weight: 52 kDa (active form), 120 kDa (precursor)

Gene ID: 4791

UniProt: Q00653

Pathways: Toll-Like Receptors Cascades