SRSF1 antibody (N-Term)

Catalog No. ABIN3043296

Product Details anti-SRSF1 Antibody

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Binding Specificity: AA 6-33, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-SF2/SRSF1 Antibody Picoband®

Sequence: VIRGPAGNND CRIYVGNLPP DIRTKDIE

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SF2/SRSF1 Antibody Picoband® (ABIN3043296). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor)". 2. Michlewski G, Sanford JR, Cáceres JF (April 2008). "The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1". Mol. Cell 30 (2): 179-89. 3. Zuo P, Manley JL (December 1993)."Functional domains of the human splicing factor ASF/SF2". EMBO J. 12 (12): 4727-37.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Alternative Name: SRSF1 (SRSF1 Products)

Background:

Synonyms: Serine/arginine-rich splicing factor 1,Alternative-splicing factor 1,ASF-1,Splicing factor, arginine/serine-rich 1,pre-mRNA-splicing factor SF2, P33 subunit,SRSF1,ASF, SF2, SF2P33, SFRS1,OK/SW-cl.3,

Tissue Specificity: Mainly expressed in the developing central nervous system.

Background: SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.

Sequence Similarities: Belongs to the splicing factor SR family.

Molecular Weight: 28 kDa

Gene ID: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization