L-Selectin antibody (AA 39-372)

Catalog No. ABIN3043326

This anti-L-Selectin antibody is a Rabbit Polyclonal antibody detecting L-Selectin in WB, IHC and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for L-Selectin antibody (AA 39-372) (ABIN3043326)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-L-Selectin Antibody

Binding Specificity: AA 39-372

Purpose: Anti-CD62L/SELL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD62L/SELL Antibody Picoband® (ABIN3043326). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human L-selectin recombinant protein (Position: W39-Y372). Human L-selectin shares 77.5 % and 78.7 % amino acid (aa) sequence identity with mouse and rat L-selectin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: SELL selectin L (lymphocyte adhesion molecule 1)". 2. Cotran, Kumar, Collins (1998). Robbins Pathologic Basis of Disease. Philadelphia: W.B Saunders Company.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: SELL

Background:

Synonyms: L-selectin,CD62 antigen-like family member L,Leukocyte adhesion molecule 1,LAM-1,Leukocyte surface antigen Leu-8,Leukocyte-endothelial cell adhesion molecule 1,LECAM1,Lymph node homing receptor,TQ1,gp90-MEL,CD62L,SELL,LNHR, LYAM1,

Tissue Specificity: Expressed in B-cell lines and T-lymphocytes. .

Background: CD62L, also known as L-selectin, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.

Sequence Similarities: Belongs to the selectin/LECAM family.

Molecular Weight: 42 kDa

Gene ID: 6402

UniProt: P14151