SFRP2 antibody (AA 104-295)

Catalog No. ABIN3043333

This Rabbit Polyclonal antibody specifically detects SFRP2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SFRP2 antibody (AA 104-295) (ABIN3043333)

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SFRP2 Antibody

Binding Specificity: AA 104-295

Purpose: Anti-SFRP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SFRP2 Antibody Picoband® (ABIN3043333). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SFRP2 recombinant protein (Position: L104-C295). Human SFRP2?shares?99%?amino?acid?(aa)?sequence?identity?with mouse SFRP2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, -
ELISA, 0.1-0.5 μg/mL, -
1. Hao XW, et al. Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma. World J Gastroenterol, 2012 Feb 14. 2. O'Hurley G, et al. The role of secreted frizzled-related protein 2 expression in prostate cancer. Histopathology, 2011 Dec. 3. Sui C, et al. Variation risks of SFRP2 hypermethylation between precancerous disease and colorectal cancer. Tumour Biol, 2014 Oct.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SFRP2

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Alternative Name: SFRP2

Background:

Synonyms: Secreted frizzled-related protein 2,FRP-2,sFRP-2,Secreted apoptosis-related protein 1,SARP-1,SFRP2,FRP2, SARP1,FKSG12, UNQ361/PRO697,

Tissue Specificity: Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. .

Background: SFRP2 is also known as FRP-2, SARP1 or SDF-5. It is Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.?

Sequence Similarities: Belongs to the secreted frizzled-related protein (sFRP) family.

Molecular Weight: 33 kDa

Gene ID: 6423

Pathways: WNT Signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation