SMC3 antibody (C-Term)

Catalog No. ABIN3043343

The Rabbit Polyclonal anti-SMC3 antibody has been validated for WB and IHC. It is suitable to detect SMC3 in samples from Human, Mouse and Rat.

Quick Overview for SMC3 antibody (C-Term) (ABIN3043343)

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMC3 Antibody

Binding Specificity: AA 1178-1216, C-Term

Purpose: Anti-SMC3 Antibody Picoband®

Sequence: ELLESADKFY GVKFRNKVSH IDVITAEMAK DFVEDDTTH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMC3 Antibody Picoband® (ABIN3043343). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SMC3, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: SMC3 structural maintenance of chromosomes 3". 2. Ghiselli G, Coffee N, Munnery CE, Koratkar R, Siracusa LD (2003). "The cohesin SMC3 is a target the for beta-catenin/TCF4 transactivation pathway".J. Biol. Chem. 278 (22): 20259-67. 3. Patel CA, Ghiselli G (2005). "Hinderin, a five-domains protein including coiled-coil motifs that binds to SMC3". BMC Cell Biol. 6 (1): 3.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMC3

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Alternative Name: SMC3

Background:

Synonyms: Structural maintenance of chromosomes protein 3,SMC protein 3,SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP,SMC3,BAM, BMH, CSPG6, SMC3L1,

Tissue Specificity: Predominantly expressed in the brain.

Background: Structural maintenance of chromosomes 3, also known as SMC3, is a human gene. This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.

Molecular Weight: 140 kDa

Gene ID: 9126

Pathways: Stem Cell Maintenance