Sp3 antibody (C-Term)

Catalog No. ABIN3043350

This anti-Sp3 antibody is a Rabbit Polyclonal antibody detecting Sp3 in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for Sp3 antibody (C-Term) (ABIN3043350)

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sp3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Sp3 Antibody

Binding Specificity: AA 569-599, C-Term

Purpose: Anti-SP3 Antibody Picoband®

Sequence: NTNDLTHLRV QVVDEEGDQQ HQEGKRLRRV A

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SP3 Antibody Picoband® (ABIN3043350). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SP3, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: SP3 Sp3 transcription factor". 2. Sun, Jian-Min, Chen Hou Yu, Moniwa Mariko, Litchfield David W, Seto Edward, Davie James R (September 2002). "The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2". J. Biol. Chem.(United States) 277 (39): 35783-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Sp3

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Alternative Name: SP3

Background:

Synonyms: Transcription factor Sp3,SPR-2,SP3,

Tissue Specificity: Ubiquitously expressed.

Background: Sp3 transcription factor, also known as SP3, refers to both a protein and the gene it is encoded by. This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. And this protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

Sequence Similarities: Belongs to the Sp1 C2H2-type zinc-finger protein family.

Molecular Weight: 82 kDa

Gene ID: 6670

UniProt: Q02447