PLD1 antibody (AA 1-330)

Catalog No. ABIN3043383

This anti-PLD1 antibody is a Rabbit Polyclonal antibody detecting PLD1 in WB. Suitable for Human and Mouse.

Quick Overview for PLD1 antibody (AA 1-330) (ABIN3043383)

Target: PLD1 (Phospholipase D1 (PLD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLD1 Antibody

Binding Specificity: AA 1-330

Purpose: Anti-Phospholipase D1/PLD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Phospholipase D1/PLD1 Antibody Picoband® (ABIN3043383). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. "Entrez Gene: PLD1 phospholipase D1, phosphatidylcholine-specific" 2. Colley, W. C., Altshuller, Y. M., Sue-Ling, C. K., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Branch, K. D., Tsirka, S. E., Bollag, R. J., Bollag, W. B., Frohman, M. A. Cloning and expression analysis of murine phospholipase D1. Biochem. J. 326: 745-753, 1997. 3. Park SH, Chun YH, Ryu SH, Suh PG, Kim H (February 1999). "Assignment of human PLD1 to human chromosome band 3q26 by fluorescence in situ hybridization". Cytogenet Cell Genet82 (3-4): 224.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PLD1

Target: PLD1 (Phospholipase D1 (PLD1))

Alternative Name: PLD1

Background:

Synonyms: Phospholipase D1,PLD 1,hPLD1,3.1.4.4,Choline phosphatase 1,Phosphatidylcholine-hydrolyzing phospholipase D1,PLD1,

Tissue Specificity: Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. .

Background: By somatic cell hybrid analysis, PLD1 is mapped to 3q26.31. This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. And PLD1 disrupted association of gamma-secretase protein components, independent of PLD1catalytic activity. What's more, PLD1 regulates intracellular trafficking of beta-amyloid, distinct from its effect on gamma-secretase activity.

Sequence Similarities: Belongs to the phospholipase D family.

Molecular Weight: 124 kDa

Gene ID: 5337

UniProt: Q13393