PD-L1 antibody (AA 45-290)

Catalog No. ABIN3043392

This anti-PD-L1 antibody is a Rabbit Polyclonal antibody detecting PD-L1 in WB and FACS. Suitable for Human.

Quick Overview for PD-L1 antibody (AA 45-290) (ABIN3043392)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 45-290

Purpose: Anti-PD-L1/CD274 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PD-L1/ Antibody Picoband® (ABIN3043392). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Barber, D. L., Wherry, E. J., Masopust, D., Zhu, B., Allison, J. P., Sharpe, A. H., Freeman, G. J., Ahmed, R. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature 439: 682-687, 2006. 2. Dong, H., Zhu, G., Tamada, K., Chen, L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.Nature Med. 5: 1365-1369, 1999. 3. Curiel, T. J., Wei, S., Dong, H., Alvarez, X., Cheng, P., Mottram, P., Krzysiek, R., Knutson, K. L., Daniel, B., Zimmermann, M. C., David, O., Burow, M., and 10 others. Blockade of B7-H1 improves myeloid dendritic cell-mediated antitumor immunity. Nature Med. 9: 562-567, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background:

Synonyms: Programmed cell death 1 ligand 1,PD-L1,PDCD1 ligand 1,Programmed death ligand 1,B7 homolog 1,B7-H1,CD274,CD274,B7H1, PDCD1L1, PDCD1LG1, PDL1,

Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. .

Background: Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40 kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What's more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.

Sequence Similarities: Belongs to the immunoglobulin superfamily. BTN/MOG family.

Molecular Weight: 40-50 kDa

Gene ID: 29126

Pathways: Cancer Immune Checkpoints