Galectin 3 antibody (AA 139-250)

Catalog No. ABIN3043399

This Rabbit Polyclonal antibody specifically detects Galectin 3 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for Galectin 3 antibody (AA 139-250) (ABIN3043399)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Galectin 3 Antibody

Binding Specificity: AA 139-250

Purpose: Anti-Galectin 3/LGALS3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Galectin 3/LGALS3 Antibody Picoband® (ABIN3043399). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Dumic J, Dabelic S, Flögel M. (2006). "Galectin-3: an open-ended story.". BBA General Subjects 1760(4): 616-635. 2. Liu F, Patterson RJ, Wang JL. (2002). "Intracellular function of galectins.". BBA General Subjects 1572 (2-3): 263-273. 3. Cooper D. (2002). "Galectinomics: finding themes in complexity.". BBA General Subjects 1572 (2-3): 209-231. 4. Raimond J, Zimonjic DB, Mignon C, et al. (1997). "Mapping of the galectin-3 gene (LGALS3) to human chromosome 14 at region 14q21-22.". Mamm. Genome 8 (9): 706-707.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: LGALS3

Background:

Synonyms: Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2;

Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .

Background: Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Sequence Similarities: Contains 1 galectin domain.

Molecular Weight: 26 kDa

Gene ID: 3958

UniProt: P17931

Pathways: RTK Signaling