MLH1 antibody (C-Term)

Catalog No. ABIN3043404

This anti-MLH1 antibody is a Rabbit Polyclonal antibody detecting MLH1 in WB. Suitable for Human.

Quick Overview for MLH1 antibody (C-Term) (ABIN3043404)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MLH1 Antibody

Binding Specificity: AA 722-756, C-Term

Purpose: Anti-MLH1 Antibody Picoband®

Sequence: KALRSHILPP KHFTEDGNIL QLANLPDLYK VFERC

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MLH1 Antibody Picoband® (ABIN3043404). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MLH1, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Tawfik HM, El-Maqsoud NM, Hak BH, El-Sherbiny YM (2011). "Head and neck squamous cell carcinoma: mismatch repair immunohistochemistry and promoter hypermethylation of hMLH1 gene". Am J Otolaryngol 32 (6): 528-36. 2. "Entrez Gene: MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background:

Synonyms: DNA mismatch repair protein Mlh1,MutL protein homolog 1,MLH1,COCA2,

Tissue Specificity: Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.

Background: MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) is a protein that in humans is encoded by the MLH1 gene located on Chromosome 3. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

Molecular Weight: 85 kDa

Gene ID: 4292

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response