STXBP1 antibody (N-Term)

Catalog No. ABIN3043413

This anti-STXBP1 antibody is a Rabbit Polyclonal antibody detecting STXBP1 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for STXBP1 antibody (N-Term) (ABIN3043413)

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-STXBP1 Antibody

Binding Specificity: AA 184-216, N-Term

Purpose: Anti-Munc18-1/STXBP1 Antibody Picoband®

Sequence: KEYPAVRYRG EYKDNALLAQ LIQDKLDAYK ADD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Munc18-1/STXBP1 Antibody Picoband® (ABIN3043413). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Munc18-1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: STXBP1 syntaxin binding protein 1". 2. Fisher, R. J., Pevsner, J., Burgoyne, R. D. Control of fusion pore dynamics during exocytosis by Munc18. Science 291: 875-878, 2001. 3. Swanson DA, Steel JM, Valle D (Jun 1998). "Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release". Genomics 48 (3): 373-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for STXBP1

Target: STXBP1 (Syntaxin Binding Protein 1 (STXBP1))

Alternative Name: STXBP1

Background:

Synonyms: Syntaxin-binding protein 1,MUNC18-1,N-Sec1,Protein unc-18 homolog 1,Unc18-1,Protein unc-18 homolog A,Unc-18A,p67,STXBP1,UNC18A,

Tissue Specificity: Brain and spinal cord. Highly enriched in axons.

Background: Syntaxin-binding protein 1, also known as Munc18-1, is a protein that in humans is encoded by the STXBP1 gene. By fluorescence in situ hybridization, the STXBP1 gene is mapped to chromosome 9q34.1. This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.

Molecular Weight: 70 kDa

Gene ID: 6812

UniProt: P61764

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport