RRM2 antibody (N-Term)

Catalog No. ABIN3043425

This anti-RRM2 antibody is a Rabbit Polyclonal antibody detecting RRM2 in WB and IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for RRM2 antibody (N-Term) (ABIN3043425)

Target: RRM2 (Ribonucleotide Reductase M2 (RRM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RRM2 Antibody

Binding Specificity: AA 1-33, N-Term

Purpose: Anti-RRM2 Antibody Picoband®

Sequence: MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENT

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RRM2 Antibody Picoband® (ABIN3043425). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RRM2, different from the related mouse and rat sequences by eight amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: ribonucleotide reductase M2". 2. Pavloff, N., Rivard, D., Masson, S., Shen, S.-H., Mes-Masson, A.-M. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. 3. Yang-Feng, T. L., Thelander, L., Lewis, W. H., Srinivasan, P. R., Francke, U. Gene localization of the ribonucleotide reductase M2 subunit and of related and co-amplified sequences on human and mouse chromosomes. (Abstract) Am. J. Hum. Genet. 39: A174 only, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-RRM2 antibody (ABIN3043425)

Xu, Li, Tao, Li, Fang, Zhao, Li, Li, Wang, Feng, Pan: "Histone acetyltransferase inhibitor II induces apoptosis in glioma cell lines via the p53 signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 33, pp. 108, (2015) (PubMed).

Target Details for RRM2

Target: RRM2 (Ribonucleotide Reductase M2 (RRM2))

Alternative Name: RRM2

Background:

Synonyms: Ribonucleoside-diphosphate reductase subunit M2,1.17.4.1,Ribonucleotide reductase small chain,Ribonucleotide reductase small subunit,RRM2,RR2,

Tissue Specificity: Overexpressed in bladder cancer cells (PubMed:17409436). .

Background: Ribonucleoside-diphosphate reductase subunit M2, also known as ribonucleotide reductase small subunit, is an enzyme that in humans is encoded by the RRM2 gene. It is mapped to 2p25-p24. This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms which differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.

Molecular Weight: 50 kDa

Gene ID: 6241

UniProt: P31350

Pathways: Mitotic G1-G1/S Phases