RAB10 antibody (C-Term)

Catalog No. ABIN3043448

The Rabbit Polyclonal anti-RAB10 antibody has been validated for WB. It is suitable to detect RAB10 in samples from Human, Mouse and Rat.

Quick Overview for RAB10 antibody (C-Term) (ABIN3043448)

Target: RAB10 (RAB10, Member RAS Oncogene Family (RAB10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAB10 Antibody

Binding Specificity: AA 153-185, C-Term

Purpose: Anti-RAB10 Antibody Picoband®

Sequence: AKANINIEKA FLTLAEDILR KTPVKEPNSE NVD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB10 Antibody Picoband® (ABIN3043448). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB10, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: RAB10 RAB10, member RAS oncogene family". 2. Chen YT, Holcomb C, Moore HP (Aug 1993). "Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag". Proc Natl Acad Sci U S A 90 (14): 6508-12. 3. He, H., Dai, F., Yu, L., She, X., Zhao, Y., Jiang, J., Chen, X., Zhao, S. Identification and characterization of nine novel human small GTPases showing variable expressions in liver cancer tissues. Gene Expr. 10: 231-242, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB10

Target: RAB10 (RAB10, Member RAS Oncogene Family (RAB10))

Alternative Name: RAB10

Background:

Synonyms: Ras-related protein Rab-10,RAB10,

Tissue Specificity: Expressed in breast, placenta, kidney, liver and pancreas. .

Background: Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis. It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs. Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes. Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.

Molecular Weight: 23 kDa

Gene ID: 10890

UniProt: P61026

Pathways: Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome