rala antibody (C-Term)

Catalog No. ABIN3043449

This anti-rala antibody is a Rabbit Polyclonal antibody detecting rala in WB. Suitable for Human, Mouse and Rat.

Quick Overview for rala antibody (C-Term) (ABIN3043449)

Target: rala (Ras-related Protein Ral-A (rala))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This rala antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-rala Antibody

Binding Specificity: AA 165-203, C-Term

Purpose: Anti-RALA Antibody Picoband®

Sequence: DKVFFDLMRE IRARKMEDSK EKNGKKKRKS LAKRIRERC

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RALA Antibody Picoband® (ABIN3043449). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RALA, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: RALA v-ral simian leukemia viral oncogene homolog A (ras related)". 2. Kashatus DF (Sep 2013). "Ral GTPases in tumorigenesis: emerging from the shadows". Experimental Cell Research 319 (15): 2337-42. 3. Rousseau-Merck MF, Bernheim A, Chardin P, Miglierina R, Tavitian A, Berger R (Jun 1988). "The ras-related ral gene maps to chromosome 7p15-22". Human Genetics 79 (2): 132-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for rala

Target: rala (Ras-related Protein Ral-A (rala))

Alternative Name: RALA

Background:

Synonyms: Ras-related protein Ral-A,RALA,RAL,

Tissue Specificity: Cells of the B- and T-lymphocyte lineages.

Background: Ras-related protein Ral-A (RalA) is a protein that in humans is encoded by the RALA gene on chromosome 7. This protein is one of two isoforms of the Ral protein, the other being RalB, and part of the Ras GTPase family. As a Ras GTPase, RalA functions as a molecular switch that becomes active when bound to GTP and inactive when bound to GDP. It can be activated by RalGEFs and, in turn, activate effectors in signal transduction pathways leading to biological outcomes. Additionally, Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer. While the above functions appear to be shared between the two Ral isoforms, their differential subcellular localizations result in their differing involvement in certain biological processes. In particular, RalA is more involved in anchorage-independent cell growth, vesicle trafficking, and cytoskeletal organization. Moreover, RalA specifically interacts with Exo84 and Sec5 to regulate transport of membrane proteins in polarized epithelial cells and GLUT4 to the plasma membrane, as well as mitochondrial fission for cell division.

Molecular Weight: 24 kDa

Gene ID: 5898

UniProt: P11233

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome