CD82 antibody (AA 98-267)

Catalog No. ABIN3043486

Product Details anti-CD82 Antibody

Target: CD82

Binding Specificity: AA 98-267

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD82 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-CD82 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD82 Antibody Picoband® (ABIN3043486). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Dong, J.-T., Lamb, P. W., Rinker-Schaeffer, C. W., Vukanovic, J., Ichikawa, T., Isaacs, J. T., Barrett, J. C. : KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886, 1995. 2. Mashimo, T., Watabe, M., Hirota, S., Hosobe, S., Miura, K., Tegtmeyer, P. J., Rinker-Shaeffer, C. W., Watabe, K. : The expression of the KAI1 gene, a tumor metastasis suppressor, is ly activated by p53. Proc. Nat. Acad. Sci. 95: 11307-11311, 1998. 3. Miyazaki, T., Kato, H., Shitara, Y., Yoshikawa, M., Tajima, K., Masuda, N., Shouji, H., Tsukada, K., Nakajima, T., Kuwano, H. : Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer 89: 955-962, 2000. 4. Jee, B. K., Park, K. M., Surendran, S., Lee, W. K., Han, C. W., Kim, Y. S., Lim, Y. : KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line. Biochem. Biophys. Res. Commun. 342: 655-661, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD82

Target: CD82

Alternative Name: CD82 (CD82 Products)

Background:

Synonyms: CD82 antigen,C33 antigen,IA4,Inducible membrane protein R2,Metastasis suppressor Kangai-1,Suppressor of tumorigenicity 6 protein,Tetraspanin-27,Tspan-27,CD82,CD82,KAI1, SAR2, ST6, TSPAN27,

Tissue Specificity: Lymphoid specific.

Background: CD82 (Cluster of Differentiation 82), also named KAI1, is a protein that in humans encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma (ESCC). And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.

Sequence Similarities: Belongs to the tetraspanin (TM4SF) family.

Molecular Weight: 30-36 kDa

Gene ID: 3732

UniProt: P27701

Pathways: p53 Signaling