BIRC3 antibody (AA 1-191)

Catalog No. ABIN3043510

This Rabbit Polyclonal antibody specifically detects BIRC3 in WB. It exhibits reactivity toward Human.

Quick Overview for BIRC3 antibody (AA 1-191) (ABIN3043510)

Target: BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIRC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BIRC3 Antibody

Binding Specificity: AA 1-191

Purpose: Anti-cIAP2/BIRC3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-cIAP2/BIRC3 Antibody Picoband® (ABIN3043510). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human cIAP2 recombinant protein (Position: M1-K191). Human cIAP2 shares 69.8% amino acid (aa) sequence identity with mouse cIAP2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: BIRC3 baculoviral IAP repeat-containing 3". 2. Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A, Korneluk RG (February 1996). "Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes". Nature 379 (6563): 349-53. 3. Rothe M, Pan MG, Henzel WJ, Ayres TM, Goeddel DV (February 1996). "The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins". Cell 83 (7): 1243-52.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BIRC3

Target: BIRC3 (Baculoviral IAP Repeat Containing 3 (BIRC3))

Alternative Name: BIRC3

Background:

Synonyms: Baculoviral IAP repeat-containing protein 3,6.3.2.-,Apoptosis inhibitor 2,API2,C-IAP2,IAP homolog C,Inhibitor of apoptosis protein 1,IAP-1,hIAP-1,hIAP1,RING finger protein 49,TNFR2-TRAF-signaling complex protein 1,BIRC3,API2, IAP1, MIHC, RNF49,

Tissue Specificity: Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.

Background: Baculoviral IAP repeat-containing protein 3 (also known as cIAP2) is a protein that in humans is encoded by the BIRC3 gene. cIAP2 is a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.

Sequence Similarities: Belongs to the IAP family.

Molecular Weight: 68 kDa

Gene ID: 330

UniProt: Q13489

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades