Adiponectin Receptor 1 antibody (N-Term)

Catalog No. ABIN3043520

The Rabbit Polyclonal anti-Adiponectin Receptor 1 antibody has been validated for WB, ICC and IF. It is suitable to detect Adiponectin Receptor 1 in samples from Human and Rat.

Quick Overview for Adiponectin Receptor 1 antibody (N-Term) (ABIN3043520)

Target: Adiponectin Receptor 1 (ADIPOR1)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adiponectin Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Adiponectin Receptor 1 Antibody

Binding Specificity: AA 51-78, N-Term

Purpose: Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband®

Sequence: EQTCPVPQEE EEEVRVLTLP LQAHHAME

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband® (ABIN3043520). Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ADIPOR1, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. "Entrez Gene: ADIPOR1 adiponectin receptor 1" 2. Yamauchi T, Kamon J, Ito Y, Tsuchida A, Yokomizo T, Kita S, Sugiyama T, Miyagishi M, Hara K, Tsunoda M, Murakami K, Ohteki T, Uchida S, Takekawa S, Waki H, Tsuno NH, Shibata Y, Terauchi Y, Froguel P, Tobe K, Koyasu S, Taira K, Kitamura T, Shimizu T, Nagai R, Kadowaki T (June 2003). "Cloning of adiponectin receptors that mediate antidiabetic metabolic effects". Nature 423 (6941): 762-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Adiponectin Receptor 1

Target: Adiponectin Receptor 1 (ADIPOR1)

Alternative Name: ADIPOR1

Background:

Synonyms: Adiponectin receptor protein 1,Progestin and adipoQ receptor family member I,ADIPOR1,PAQR1 , TESBP1A,CGI-45,

Tissue Specificity: Widely expressed (PubMed:16044242). Highly expressed in heart and skeletal muscle (PubMed:12802337). Expressed at intermediate level in brain, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes (PubMed:12802337). Weakly expressed in colon, thymus and small intestine (PubMed:12802337). .

Background: ADIPOR1 is known as Adiponectin receptor protein 1. This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene.

Sequence Similarities: Belongs to the ADIPOR family.

Molecular Weight: 43 kDa

UniProt: Q96A54

Pathways: AMPK Signaling