Aquaporin 1 antibody (C-Term)

Catalog No. ABIN3043524

This anti-Aquaporin 1 antibody is a Rabbit Polyclonal antibody detecting Aquaporin 1 in WB, IHC, IF and FACS. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 6+ publications.

Quick Overview for Aquaporin 1 antibody (C-Term) (ABIN3043524)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Aquaporin 1 Antibody

Binding Specificity: AA 240-269, C-Term

Purpose: Anti-Aquaporin 1/AQP1 Antibody Picoband®

Sequence: DRVKVWTSGQ VEEYDLDADD INSRVEMKPK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aquaporin 1/AQP1 Antibody Picoband® (ABIN3043524). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 1, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human1. Denker, B. M., Smith, B. L., Kuhajda, F. P., Agre, P. : Identification, purification, and partial characterization of a novel M(r) 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 263: 15634-15642, 1988. 2. Thiagarajah, J. R., Verkman, A. S. : Aquaporin deletion in mice reduces corneal water permeability and delays restoration of transparency after swelling. J. Biol. Chem. 277: 19139-19144, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Aquaporin 1 antibody (ABIN3043524)

Zhang, Chen, Dong, Shi: "Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells." in: Oncology letters, Vol. 15, Issue 3, pp. 3864-3869, (2018) (PubMed).

Li, Zhao, Miao, Xu, Xiao, Liu: "Dynamic expression and roles of sequestome‑1/p62 in LPS‑induced acute kidney injury in mice." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7618-7626, (2018) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Huo, Liu, Wang, Zhang, Wang, Yang, Sun, Xu: "Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury." in: Neural regeneration research, Vol. 7, Issue 22, pp. 1729-35, (2015) (PubMed).

Zhang, Wu, Yang, Liu, Liu: "Effects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6466-73, (2015) (PubMed).

Ran, Wang, Chen, Zeng, Zhou, Zheng, Sun, Wang, Lv, Liang, Zhang, Liu: "Aquaporin-1 expression and angiogenesis in rabbit chronic myocardial ischemia is decreased by acetazolamide." in: Heart and vessels, Vol. 25, Issue 3, pp. 237-47, (2010) (PubMed).

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: AQP1

Background:

Synonyms: Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28,

Tissue Specificity: Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. .

Background: Aquaporin 1 is a 28-kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient.

Sequence Similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family.

Molecular Weight: 25, 35-38 kDa

Gene ID: 358

UniProt: P29972

Pathways: Hormone Transport