AGFG1 antibody (N-Term)

Catalog No. ABIN3043530

This anti-AGFG1 antibody is a Rabbit Polyclonal antibody detecting AGFG1 in WB, IF, ICC and FACS. Suitable for Human and Rat.

Quick Overview for AGFG1 antibody (N-Term) (ABIN3043530)

Target: AGFG1 (HRB) (HIV-1 Rev Binding Protein (HRB))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGFG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-AGFG1 Antibody

Binding Specificity: AA 5-28, N-Term

Purpose: Anti-AGFG1 Antibody Picoband®

Sequence: AKRKQEEKHL KMLRDMTGLP HNRK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-AGFG1 Antibody (ABIN3043530). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: HRB HIV-1 Rev binding protein". 2. Fritz CC, Zapp ML, Green MR (Sep 1995). "A human nucleoporin-like protein that specifically interacts with HIV Rev".Nature 376 (6540): 530-3.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AGFG1

Target: AGFG1 (HRB) (HIV-1 Rev Binding Protein (HRB))

Alternative Name: AGFG1

Background:

Synonyms: Arf-GAP domain and FG repeat-containing protein 1,HIV-1 Rev-binding protein,Nucleoporin-like protein RIP,Rev-interacting protein,Rev/Rex activation domain-binding protein,AGFG1,HRB, RAB, RIP,

Tissue Specificity: Ubiquitously expressed. .

Background: Arf-GAP domain and FG repeats-containing protein 1 is aprotein that in humans is encoded by the AGFG1 gene. AGFG1 is located to 2q36.3. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-ed RNAs. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Sequence Similarities: Contains 1 Arf-GAP domain.

Molecular Weight: 58 kDa

Gene ID: 3267

UniProt: P52594