Prolactin antibody (AA 30-226)

Catalog No. ABIN3043552

This Rabbit Polyclonal antibody specifically detects Prolactin in WB and ELISA. It exhibits reactivity toward Mouse and has been mentioned in 3+ publications.

Quick Overview for Prolactin antibody (AA 30-226) (ABIN3043552)

Target: Prolactin (PRL)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prolactin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Prolactin Antibody

Binding Specificity: AA 30-226

Purpose: Anti-Prolactin/PRL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Prolactin/PRL Antibody Picoband® (ABIN3043552). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Prolactin recombinant protein (Position: L30-C226). Mouse Prolactin shares 59.8% and 84.8% amino acid (aa) sequence identity with human and rat Prolactin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse , -
ELISA, 0.1-0.5 μg/mL, -
1. Cooke, N. E., Baxter, J. D.Structural analysis of the prolactin gene suggests a separate origin for its 5-prime end.Nature 297: 603-606, 1982. 2. Ling, C., Svensson, L., Oden, B., Weijdegard, B., Eden, B., Eden, S., Billig, H.Identification of functional prolactin (PRL) receptor gene expression: PRL inhibits lipoprotein lipase activity in human white adipose tissue.J. Clin. Endocr. Metab. 88: 1804-1808, 2003. 3. Shingo, T., Gregg, C., Enwere, E., Fujikawa, H., Hassam, R., Geary, C., Cross, J. C., Weiss, S.Pregnancy-stimulated neurogenesis in the adult female forebrain mediated by prolactin. Science 299: 117-120, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Prolactin antibody (ABIN3043552)

Wang, Tan, Ma, Liang, Zhang, Tan, Wang, Luo: "Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice." in: Nutrients, Vol. 8, Issue 11, (2017) (PubMed).

Huang, Cao, Liu, Zou, Li, Yin: "MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro." in: International journal of clinical and experimental pathology, Vol. 6, Issue 10, pp. 2129-36, (2014) (PubMed).

Huang, Wang, Zou, Liu, Cao, Yin: "Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with endometriosis in vitro." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2487-96, (2013) (PubMed).

Target Details for Prolactin

Target: Prolactin (PRL)

Alternative Name: Prl

Background:

Synonyms: Prolactin,PRL,Prl,

Tissue Specificity: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Background: Prolactin (PRL) also known as luteotropic hormone (LTH) is a protein that in humans is encoded by the PRL gene. Prolactin is a peptide hormone discovered by Henry Friesen. Although it is perhaps best known for its role in lactation, prolactin already existed in the oldest known vertebrates-fishes-where its most important functions were probably related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Sequence Similarities: Belongs to the synaptophysin/synaptobrevin family.

Molecular Weight: 10 kDa

Gene ID: 19109

UniProt: P06879

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus