PSCA antibody (AA 21-95)

Catalog No. ABIN3043554

This anti-PSCA antibody is a Rabbit Polyclonal antibody detecting PSCA in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PSCA antibody (AA 21-95) (ABIN3043554)

Target: PSCA (Prostate Stem Cell Antigen (PSCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSCA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSCA Antibody

Binding Specificity: AA 21-95

Purpose: Anti-PSCA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PSCA Antibody Picoband® (ABIN3043554). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Hillerdal V, et al. Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice. BMC Cancer, 2014 Jan 18. 2. Taeb J, et al. Expression of prostate stem cell antigen (PSCA) in prostate cancer: a tissue microarray study of Iranian patients. Pathol Res Pract, 2014 Jan.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PSCA antibody (ABIN3043554)

Wang, Wu, Zhu, Ding, Cai, Ni, Wu, Meng, Zhang, Zhang, Li, Wang, Chen, Jin, Zhang: "PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population." in: Scientific reports, Vol. 6, pp. 23465, (2017) (PubMed).

Zhao, Ma, Zeng, Qi, Ou, Liang: "Small interference RNA-mediated silencing of prostate stem cell antigen attenuates growth, reduces migration and invasion of human prostate cancer PC-3M cells." in: Urologic oncology, Vol. 31, Issue 3, pp. 343-51, (2013) (PubMed).

Target Details for PSCA

Target: PSCA (Prostate Stem Cell Antigen (PSCA))

Alternative Name: PSCA

Background:

Synonyms: Prostate stem cell antigen,PSCA,UNQ206/PRO232,

Tissue Specificity: Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts). Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen- independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level). Not detected in normal intestinal epithelium (at protein level). .

Background: PSCA is also known as PRO232. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals, this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Contains 1 UPAR/Ly6 domain.

Molecular Weight: 25 kDa

Gene ID: 8000

UniProt: O43653