PSCA antibody (AA 21-95)

Catalog No. ABIN3043554

Product Details anti-PSCA Antibody

Target: PSCA (Prostate Stem Cell Antigen (PSCA))

Binding Specificity: AA 21-95

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSCA antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Prostate stem cell antigen(PSCA) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Prostate stem cell antigen(PSCA) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: prostate stem cell antigen
Protein Name: Prostate stem cell antigen

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, The detection limit for PSCA is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-PSCA antibody (ABIN3043554)

Wang, Wu, Zhu, Ding, Cai, Ni, Wu, Meng, Zhang, Zhang, Li, Wang, Chen, Jin, Zhang: "PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population." in: Scientific reports, Vol. 6, pp. 23465, (2017) (PubMed).

Zhao, Ma, Zeng, Qi, Ou, Liang: "Small interference RNA-mediated silencing of prostate stem cell antigen attenuates growth, reduces migration and invasion of human prostate cancer PC-3M cells." in: Urologic oncology, Vol. 31, Issue 3, pp. 343-51, (2013) (PubMed).

Target Details for PSCA

Target: PSCA (Prostate Stem Cell Antigen (PSCA))

Alternative Name: PSCA (PSCA Products)

Background: PSCA is also known as PRO232. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals, this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.

Synonyms: PRO 232 antibody|PRO232 antibody|Prostate stem cell antigen antibody

Gene ID: 8000

UniProt: O43653