RAB3A antibody (C-Term)

Catalog No. ABIN3043559

The Rabbit Polyclonal anti-RAB3A antibody has been validated for WB. It is suitable to detect RAB3A in samples from Human, Rat and Mouse.

Quick Overview for RAB3A antibody (C-Term) (ABIN3043559)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAB3A Antibody

Binding Specificity: AA 192-220, C-Term

Purpose: Anti-Rab3A Antibody Picoband®

Sequence: DTADPAVTGA KQGPQLSDQQ VPPHQDCAC

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Rab3A Antibody Picoband® (ABIN3043559). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab3A, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Giovedi, S., Darchen, F., Valtorta, F., Greengard, P., Benfenati, F. Synapsin is a novel Rab3 effector protein on small synaptic vesicles. II. Functional effects of the Rab3A-synapsin I interaction. J. Biol. Chem. 279: 43769-43779, 2004. 2. Huang, Y.-Y., Zakharenko, S. S., Schoch, S., Kaeser, P. S., Janz, R., Sudhof, T. C., Siegelbaum, S. A., Kandel, E. R. Genetic evidence for a protein-kinase-A-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic potentiation. Proc. Nat. Acad. Sci. 102: 9365-9370, 2005. 3. Rousseau-Merck M, Zahraoui A, Bernheim A, Touchot N, Miglierina R, Tavitian A et al. (January 1990). "Chromosome mapping of the human ras-related rab3A gene to 19p13.2". Genomics 5 (4): 694-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: RAB3A

Background:

Synonyms: Ras-related protein Rab-3A,RAB3A,

Tissue Specificity: Specifically expressed in brain.

Background: Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. This gene is mapped to 19p13.11. Synapsin I stimulated the RAB3A cycle by increasing GTP binding, GTPase activity, and RAB3A recruitment to the synaptic vesicle membrane. Conversely, RAB3A inhibited synapsin I binding to actin and synapsin I-induced synaptic vesicle clustering. It has been found that RAB3A was necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, RAB3A has been found to regulate a late step in synaptic vesicle fusion.

Molecular Weight: 25 kDa

Gene ID: 5864

UniProt: P20336

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport