DKK2 antibody (AA 34-259)

Catalog No. ABIN3043565

The Rabbit Polyclonal anti-DKK2 antibody has been validated for WB. It is suitable to detect DKK2 in samples from Human and Rat.

Quick Overview for DKK2 antibody (AA 34-259) (ABIN3043565)

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DKK2 Antibody

Binding Specificity: AA 34-259

Purpose: Anti-DKK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DKK2 Antibody Picoband® (ABIN3043565). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DKK2 recombinant protein (Position: K34-I259). Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: DKK2 dickkopf homolog 2 (Xenopus laevis)". 2. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA (Dec 1999). "Functional and structural diversity of the human Dickkopf gene family". Gene 238 (2): 301-13. 3. Wu W, Glinka A, Delius H, Niehrs C (2001). "Mutual antagonism between dickkopf1 and dickkopf2 regulates Wnt/beta-catenin signalling.". Curr. Biol. 10 (24): 1611-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DKK2

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Alternative Name: DKK2

Background:

Synonyms: Dickkopf-related protein 2,Dickkopf-2,Dkk-2,hDkk-2,DKK2,UNQ682/PRO1316,

Tissue Specificity: Expressed in heart, brain, skeletal muscle and lung.

Background: Dickkopf-related protein 2 is a protein that in humans is encoded by the DKK2 gene. This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).

Sequence Similarities: Belongs to the DAXX family.

Molecular Weight: 28 kDa

Gene ID: 27123

Pathways: WNT Signaling