PAX2A antibody (C-Term)

Catalog No. ABIN3043591

This Rabbit Polyclonal antibody specifically detects PAX2A in WB and IHC. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for PAX2A antibody (C-Term) (ABIN3043591)

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PAX2A Antibody

Binding Specificity: AA 248-282, C-Term

Purpose: Anti-Pax2 Antibody Picoband®

Sequence: RKHLRADTFT QQQLEALDRV FERPSYPDVF QASEH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Pax2 Antibody Picoband® (ABIN3043591). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Pax2, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Rat, Human
1. "Entrez Gene: PAX2 paired box gene 2". 2. Pilz AJ, Povey S, Gruss P, Abbott CM (1993). "Mapping of the human homologs of the murine paired-box-containing genes". Mamm. Genome 4 (2): 78-82. 3. Pfeffer, Peter L., Bernhard Payter, Gerlinde Reim, Marina Pasca di Magliano, Meinrad Busslinger (2001). "The activation and maintenance of Pax2 expression at the mid-hindbrain boundary is controlled by separate enhancers". Development (133): 307-318.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PAX2A antibody (ABIN3043591)

Yang, He, Peng, Zhong, Deng, Yu, Guan, Zuo, Xu: "Matrine suppresses the migration and invasion of NSCLC cells by inhibiting PAX2-induced epithelial-mesenchymal transition." in: OncoTargets and therapy, Vol. 10, pp. 5209-5217, (2017) (PubMed).

Target Details for PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Alternative Name: PAX2

Background:

Synonyms: Paired box protein Pax-2,PAX2,

Tissue Specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Background: Paired box gene 2, also known as PAX2, is a protein which in humans is encoded by the PAX2 gene. This gene is mapped to 10q24. PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 45 kDa

Gene ID: 5076

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation