MYB antibody (AA 1-201)

Catalog No. ABIN3043597

This Rabbit Polyclonal antibody specifically detects MYB in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for MYB antibody (AA 1-201) (ABIN3043597)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MYB Antibody

Binding Specificity: AA 1-201

Purpose: Anti-c-Myb Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-c-Myb Antibody Picoband® (ABIN3043597). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human c-Myb recombinant protein (Position: M1-E201). Human c-Myb shares 100% amino acid (aa) sequence identity with mouse c-Myb.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Chen Y, Xu H, Liu J, Zhang C, Leutz A, Mo X (Jul 2007). "The c-Myb functions as a downstream target of PDGF-mediated survival signal in vascular smooth muscle cells". Biochem Biophys Res Commun 360 (2): 433-6. 2. Xiao, C., Calado, D. P., Galler, G., Thai, T.-H., Patterson, H. C., Wang, J., Rajewsky, N., Bender, T. P., Rajewsky, K. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131: 146-159, 2007. Xiao, C., Calado, D. P., Galler, G., Thai, T.-H., Patterson, H. C., Wang, J., Rajewsky, N., Bender, T. P., Rajewsky, K. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131: 146-159, 2007. 3. Vargova K, Curik N, Burda P, Basova P, Kulvait V, Pospisil V, Savvulidi F, Kokavec J, Necas E, Berkova A, Obrtlikova P, Karban J, Mraz M, Pospisilova S, Mayer J, Trneny M, Zavadil J, Stopka T (April 2011). "MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia". Blood 117 (14): 3816-25.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB

Background:

Synonyms: Transcriptional activator Myb,Proto-oncogene c-Myb,MYB,

Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .

Background: Myb proto-oncogene protein, also known as transcriptional activator Myb, is a protein that in humans is encoded by the MYB gene. It is a member of the MYB (myeloblastosis) family of transcription factors. This gene is mapped to 6q23.3.The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis, including the regulation of miR-155 in B-cells. MYB is also a critical target of MIR150.

Sequence Similarities: Contains 3 HTH myb-type DNA-binding domains.

Molecular Weight: 70-80 kDa

Gene ID: 4602

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process