PGK1 antibody (C-Term)

Catalog No. ABIN3043604

The Rabbit Polyclonal anti-PGK1 antibody has been validated for WB, IF, FACS and ICC. It is suitable to detect PGK1 in samples from Human, Mouse and Rat.

Quick Overview for PGK1 antibody (C-Term) (ABIN3043604)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PGK1 Antibody

Binding Specificity: AA 312-337, C-Term

Purpose: Anti-PGK1 Antibody Picoband®

Sequence: MGLDCGPESS KKYAEAVTRA KQIVWN

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PGK1 Antibody Picoband® (ABIN3043604). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PGK1, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: PGK1 phosphoglycerate kinase 1". 2. Michelson AM, Markham AF, Orkin SH (May 1983). "Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase". Proc Natl Acad Sci U S A 80 (2): 472-6. 3. Singer-Sam J, Keith DH, Tani K, Simmer RL, Shively L, Lindsay S, Yoshida A, Riggs AD (May 1985). "Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase]". Gene 32 (3): 409-17.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1

Background:

Synonyms: Phosphoglycerate kinase 1,2.7.2.3,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,PGKA,MIG10, OK/SW-cl.110,

Tissue Specificity: Ubiquitous, highest levels of expression in heart and skeletal muscle. .

Background: PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Molecular Weight: 43 kDa

Gene ID: 5230

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process