MAFA antibody (Middle Region)

Catalog No. ABIN3043609

This Rabbit Polyclonal antibody specifically detects MAFA in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for MAFA antibody (Middle Region) (ABIN3043609)

Target: MAFA (Transcription Factor MafA (MAFA))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAFA Antibody

Binding Specificity: AA 136-167, Middle Region

Purpose: Anti-Transcription factor MafA MAFA Antibody Picoband®

Sequence: EDAVEALIGS GHHGAHHGAH HPAAAAAYEA FR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Transcription factor MafA MAFA Antibody (ABIN3043609). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MAFA, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002. 2. Zhang, C., Moriguchi, T., Kajihara, M., Esaki, R., Harada, A., Shimohata, H., Oishi, H., Hamada, M., Morito, N., Hasegawa, K., Kudo, T., Engel, J. D., Yamamoto, M., Takahashi, S. MafA is a key regulator of glucose-stimulated insulin secretion. Molec. Cell. Biol. 25: 4969-4976, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAFA

Target: MAFA (Transcription Factor MafA (MAFA))

Alternative Name: MAFA

Background:

Synonyms: Transcription factor MafA,Pancreatic beta-cell-specific transcriptional activator,Transcription factor RIPE3b1,V-maf musculoaponeurotic fibrosarcoma oncogene homolog A,MAFA,

Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.

Sequence Similarities: Belongs to the bZIP family. Maf subfamily.

Molecular Weight: 37 kDa

Gene ID: 389692

Pathways: Hormone Transport