Caspase 2 antibody (C-Term)

Catalog No. ABIN3043696

This Rabbit Polyclonal antibody specifically detects Caspase 2 in WB. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 27+ publications.

Quick Overview for Caspase 2 antibody (C-Term) (ABIN3043696)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 2 Antibody

Binding Specificity: AA 417-439, C-Term

Purpose: Anti-Caspase-2/CASP2 Antibody Picoband®

Sequence: REGYAPGTEF HRCKEMSEYC STL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Caspase-2/CASP2 Antibody (ABIN3043696). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-2, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Bergeron, L., Perez, G. I., Macdonald, G., Shi, L., Sun, Y., Jurisicova, A., Varmuza, S., Latham, K. E., Flaws, J. A., Salter, J. C. M., Hara, H., Moskowitz, M. A., Li, E., Greenberg, A., Tilly, J. L., Yuan, J. : Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 12: 1304-1314, 1998. 2. Tinel, A., Tschopp, J. : The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. Science 304: 843-846, 2004.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Caspase 2 antibody (ABIN3043696)

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, (2015) (PubMed).

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, (2015) (PubMed).

Yang, Li, Liu, Shi, Zhang: "Inhibitory effect of tetramethylpyrazine preconditioning on overload training-induced myocardial apoptosis in rats." in: Chinese journal of integrative medicine, Vol. 21, Issue 6, pp. 423-30, (2015) (PubMed).

Li, Wang, Wang: "Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina." in: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB), Vol. 31, Issue 4, pp. 352-9, (2015) (PubMed).

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, (2015) (PubMed).

Peng, Chen, Chen, Fang, Cui, Zuo, Deng, Chen, Geng, Lai: "Aflatoxin B1 affects apoptosis and expression of Bax, Bcl-2, and Caspase-3 in thymus and bursa of fabricius in broiler chickens." in: Environmental toxicology, (2015) (PubMed).

Huang, Lin, Su, Ye: "Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell." in: Neurochemical research, Vol. 40, Issue 1, pp. 27-35, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Yan, Liang, Zhang, Liu, Bu: "Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL-RVG) in vitro." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 317-26, (2014) (PubMed).

Wei, Tian, Yan, Shao, Xie: "Protective effects of phillyrin on H2O 2-induced oxidative stress and apoptosis in PC12 cells." in: Cellular and molecular neurobiology, Vol. 34, Issue 8, pp. 1165-73, (2014) (PubMed).

Mao, Song, Li, Lv, Zhao, Li, Feng, Chen: "8-hydroxy-2-(di-n-propylamino)tetralin intervenes with neural cell apoptosis following diffuse axonal injury." in: Neural regeneration research, Vol. 8, Issue 2, pp. 133-42, (2014) (PubMed).

Tian, Wei, Li, Wang, Zhang, Xie: "Pathway of programmed cell death and oxidative stress induced by ?-hydroxybutyrate in dairy cow abomasum smooth muscle cells and in mouse gastric smooth muscle." in: PLoS ONE, Vol. 9, Issue 5, pp. e96775, (2014) (PubMed).

Song, Wang, Chen, Zhang, Wang, Liu, Gao, Yan: "Toxic effects of sodium fluoride on cell proliferation and apoptosis of Leydig cells from young mice." in: Journal of physiology and biochemistry, Vol. 70, Issue 3, pp. 761-8, (2014) (PubMed).

Dong, Wang: "Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts." in: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, Vol. 26, Issue 2, pp. 200-7, (2014) (PubMed).

Zhao, Li: "Altholactone induces reactive oxygen species-mediated apoptosis in bladder cancer T24 cells through mitochondrial dysfunction, MAPK-p38 activation and Akt suppression." in: Oncology reports, Vol. 31, Issue 6, pp. 2769-75, (2014) (PubMed).

Khan, Li, Ahmad Khan, Rasul, Nawaz, Sun, Zheng, Ma: "Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction." in: BioMed research international, Vol. 2013, pp. 719858, (2013) (PubMed).

Chen, Shu, Peng, Fang, Cui, Chen, Wang, Chen, Zuo, Deng, Geng, Lai: "Protective role of sodium selenite on histopathological lesions, decreased T-cell subsets and increased apoptosis of thymus in broilers intoxicated with aflatoxin B?." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 59, pp. 446-54, (2013) (PubMed).

Chen, Li, Qiu, You, Zhang, Dong, Zuo: "Kinetics of apoptosis and expression of apoptosis-related proteins in rat CA3 hippocampus cells after experimental diffuse brain injury." in: Cell biochemistry and biophysics, Vol. 67, Issue 3, pp. 1015-9, (2013) (PubMed).

Rasul, Yu, Khan, Zhang, Iqbal, Ma, Yang: "Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways." in: International journal of oncology, Vol. 40, Issue 4, pp. 1153-61, (2012) (PubMed).

Target Details for Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: CASP2

Background:

Synonyms: Caspase-2,CASP-2,3.4.22.55,Neural precursor cell expressed developmentally down-regulated protein 2,NEDD-2,Protease ICH-1,Caspase-2 subunit p18,Caspase-2 subunit p13,Caspase-2 subunit p12,CASP2,ICH1, NEDD2,

Tissue Specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Background: Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 70-74 kDa

UniProt: P42575

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway