CCR6 antibody (C-Term)

Catalog No. ABIN3043702

This Rabbit Polyclonal antibody specifically detects CCR6 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CCR6 antibody (C-Term) (ABIN3043702)

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CCR6 Antibody

Binding Specificity: AA 358-374, C-Term

Purpose: Anti-CCR6/CCR6 Antibody Picoband®

Sequence: RQTSETADND NASSFTM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CCR6/CCR6 Antibody (ABIN3043702). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CCR6, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Zaballos, A., Varona, R., Gutierrez, J., Lind, P., Marquez, G. : Molecular cloning and RNA expression of two new human chemokine receptor-like genes. Biochem. Biophys. Res. Commun. 227: 846-853, 1996. 2. Liao, F., Lee, H.-H., Farber, J. M. : Cloning of STRL22, a new human gene encoding a G-Protein-coupled receptor related to chemokine receptors and located on chromosome 6q27. Genomics 40: 175-180, 1997. 3. Homey, B., Dieu-Nosjean, M.-C., Wiesenborn, A., Massacrier, C., Pin, J.-J., Oldham, E., Catron, D., Buchanan, M. E., Muller, A., de Waal Malefyt, R., Deng, G., Orozco, R., Ruzicka, T., Lehmann, P., Lebecque, S., Caux, C., Zlotnik, A. : Up-regulation of macrophage inflammatory protein-3-alpha/CCL20 and CC chemokine receptor 6 in psoriasis. J. Immun. 164: 6621-6632, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCR6

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Alternative Name: CCR6

Background:

Synonyms: C-C chemokine receptor type 6,C-C CKR-6,CC-CKR-6,CCR-6,Chemokine receptor-like 3,CKR-L3,DRY6,G-protein coupled receptor 29,GPR-CY4,GPRCY4,LARC receptor,CD196,CCR6,CKRL3, CMKBR6, GPR29, STRL22,

Tissue Specificity: Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Background: CCR6, also termed CKRL3, encodes a 369-amino acid polypeptide with greatest similarity to the family of alpha-chemokine-binding receptors. Unlike most chemokine receptor genes, it is encoded by more than 1 exon. Weakly expressed as a 4-kb transcript in spleen, lymph nodes, peripheral blood lymphocytes and appendix, CCR6 gene is located at 6q27. As the receptor for MIP-3-alpha, its activation leads to phospholipase C-dependent intracellular Ca (2+) mobilization. Additionally, CCR6 are markedly upregulated in psoriasis.

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 120 kDa

UniProt: P51684

Pathways: cAMP Metabolic Process