HRH3 antibody (C-Term)

Catalog No. ABIN3043704

This anti-HRH3 antibody is a Rabbit Polyclonal antibody detecting HRH3 in WB. Suitable for Human, Rat and Mouse.

Quick Overview for HRH3 antibody (C-Term) (ABIN3043704)

Target: HRH3 (Histamine Receptor H3 (HRH3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRH3 Antibody

Binding Specificity: AA 428-445, C-Term

Purpose: Anti-Histamine H3 receptor HRH3 Antibody Picoband®

Sequence: CPQKLKIQPH SSLEHCWK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Histamine H3 receptor HRH3 Antibody (ABIN3043704). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HRH3, different from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Hill, S. J., Ganellin, C. R., Timmerman, H., Schwartz, J. C., Shankley, N. P., Young, J. M., Schunack, W., Levi, R., Haas, H. L. : International Union of Pharmacology. XIII. Classification of histamine receptors. Pharm. Rev. 49: 253-278, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: HRH3

Background:

Synonyms: Histamine H3 receptor,H3R,HH3R,G-protein coupled receptor 97,HRH3,GPCR97,

Tissue Specificity: Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.

Background: The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22 % and 21.4 % amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.

Molecular Weight: 49 kDa

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour