TNXB antibody (C-Term)

Catalog No. ABIN3043715

The Rabbit Polyclonal anti-TNXB antibody has been validated for IHC, WB, ICC, IF and FACS. It is suitable to detect TNXB in samples from Human.

Quick Overview for TNXB antibody (C-Term) (ABIN3043715)

Target: TNXB (Tenascin XB (TNXB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNXB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TNXB Antibody

Binding Specificity: AA 3309-3337, C-Term

Purpose: Anti-TNXB Antibody Picoband®

Sequence: DLRAVAVSGL DPARKYKFLL FGLQNGKRH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TNXB Antibody Picoband® (ABIN3043715). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TNXB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: tenascin XB". 2. Bristow, J., Tee, M. K., Gitelman, S. E., Mellon, S. H., Miller, W. L. Tenascin-X: a novel extracellular matrix protein encoded by the human XB gene overlapping P450c21B. J. Cell Biol. 122: 265-278, 1993. 3. Tee MK, Thomson AA, Bristow J, Miller WL (July 1995). "Sequences promoting the transcription of the human XA gene overlapping P450c21A correctly predict the presence of a novel, adrenal-specific, truncated form of tenascin-X". Genomics 28 (2): 171-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TNXB

Target: TNXB (Tenascin XB (TNXB))

Alternative Name: TNXB

Background:

Synonyms: Tenascin-X,TN-X,Hexabrachion-like protein,TNXB,HXBL, TNX, TNXB1, TNXB2, XB,

Tissue Specificity: Highly expressed in fetal adrenal, in fetal testis, fetal smooth, striated and cardiac muscle. Isoform XB- short is only expressed in the adrenal gland.

Background: tenascin X (TN-X), also known as hexabrachion-like protein, is a glycoprotein that is expressed in connective tissues including skin, joints and muscles. In humans, tenascin X is encoded by the TNXB gene. This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 458 kDa

Gene ID: 7148

UniProt: P22105