CALB1 antibody (AA 2-175)

Catalog No. ABIN3043771

This Rabbit Polyclonal antibody specifically detects CALB1 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CALB1 antibody (AA 2-175) (ABIN3043771)

Target: CALB1 (Calbindin (CALB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CALB1 Antibody

Binding Specificity: AA 2-175

Purpose: Anti-Calbindin/CALB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Calbindin/CALB1 Antibody Picoband® (ABIN3043771). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
1. Parmentier M., "The human calbindins: cDNA and gene cloning.",Adv. Exp. Med. Biol. 255:233-240(1989). 2. Strauss K.I., Kuznicki J., Winsky L., Kawagoe J.I., Hammer M., Jacobowitz D.M.,"The mouse calretinin gene promoter region: structural and functional components.",Brain Res. Mol. Brain Res. 49:175-187(1997). 3. Gabrielides C., McCormack A.L., Hunt D.F., Christakos S.,"Brain calbindin-D28k and an Mr 29,000 calcium binding protein in cerebellum are different but related proteins: evidence obtained from sequence analysis by tandem mass spectrometry.",Biochemistry 30:656-662(1991).

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: CALB1

Background:

Synonyms: Calbindin,Calbindin D28,D-28K,Vitamin D-dependent calcium-binding protein, avian-type,CALB1,CAB27,

Tissue Specificity: Expressed in all cell types so far analyzed.

Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Sequence Similarities: Belongs to the calbindin family.

Molecular Weight: 37 kDa

Gene ID: 793

UniProt: P05937