Caveolin-1 antibody (AA 4-178)

Catalog No. ABIN3043803

Product Details anti-Caveolin-1 Antibody

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Binding Specificity: AA 4-178

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Purpose: Anti-Caveolin-1/CAV1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Caveolin-1/CAV1 Antibody Picoband® (ABIN3043803). Tested in Flow Cytometry, IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunohistochemistry (Frozen Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human1. Tahir, S. A., Yang, G., Ebara, S., Timme, T. L., Satoh, T., Li, L., Goltsov, A., Ittmann, M., Morrisett, J. D., Thompson, T. C.Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer.Cancer Res. 61: 3882-3885, 2001. 2. Wang, X. M., Zhang, Y., Kim, H. P., Zhou, Z., Feghali-Bostwick, C. A., Liu, F., Ifedigbo, E., Xu, X., Oury, T. D., Kaminski, N., Choi, A. M. K.Caveolin-1: a critical regulator of lung fibrosis in idiopathic pulmonary fibrosis.J. Exp. Med. 203: 2895-2906, 2006. 3. Yang, G., Truong, L. D., Timme, T. L., Ren, C., Wheeler, T. M., Park, S. H., Nasu, Y., Bangma, C. H., Kattan, M. W., Scardino, P. T., Thompson, T. C.Elevated expression of caveolin is associated with prostate and breast cancer.Clin. Cancer Res. 4: 1873-1880, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Caveolin-1 antibody (ABIN3043803)

Shi, Li, Jia, Ji, Song, Cheng, Wu, Song, Zhang, Zhu, Yang: "MicroRNA-199a-5p affects porcine preadipocyte proliferation and differentiation." in: International journal of molecular sciences, Vol. 15, Issue 5, pp. 8526-38, (2015) (PubMed).

Wang, Zhao, Wu, He, Qu, Zhao, Zhao, Li, Wang: "Mechanistic analysis of taxol-induced multidrug resistance in an ovarian cancer cell line." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 14, Issue 9, pp. 4983-8, (2013) (PubMed).

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1 (CAV1 Products)

Background:

Synonyms: Caveolin-1,CAV1,CAV,

Tissue Specificity: Expressed in muscle and lung, less so in liver, brain and kidney.

Background: CAV1 ( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

Sequence Similarities: Belongs to the caveolin family.

Molecular Weight: 22 kDa

Gene ID: 857

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals