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CYP1A2 antibody (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2) (AA 185-320) Primary Antibody

CYP1A2 Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (2)
Catalog No. ABIN3043820
$280.00
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target
    CYP1A2
    Binding Specificity
    • 16
    • 11
    • 10
    • 8
    • 8
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 185-320
    Reactivity
    Human, Mouse, Rat
    Host
    • 58
    • 18
    • 8
    Rabbit
    Clonality
    • 69
    • 15
    Polyclonal
    Conjugate
    • 42
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CYP1A2 antibody is un-conjugated
    Application
    • 59
    • 43
    • 31
    • 23
    • 22
    • 13
    • 13
    • 8
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: cytochrome P450, family 1, subfamily A, polypeptide 2
    Protein Name: Cytochrome P450 1A2
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Fan, Jiang, Wang, Tan, Zeng, Wang, Chen, Qu, Gonzalez, Huang, Bi: "Wuzhi tablet (Schisandra Sphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 12, pp. 1982-90, 2014 (PubMed).

    Jiang, Fan, Wang, Chen, Zeng, Tan, Gonzalez, Huang, Bi: "Schisandrol B protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of liver regeneration." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 143, Issue 1, pp. 107-15, 2014 (PubMed).

  • Target
    CYP1A2
    Alternative Name
    CYP1A2 (CYP1A2 Antibody Abstract)
    Synonyms
    CP12, P3-450, P450(PA), CYP1A2, CYPIA2, P450-PM4, Cyp1a1, P450-3, CYPD45, P-450d, RATCYPD45, CYP1A5, CP450, CYP-IA2, cytochrome P450 family 1 subfamily A member 2, cytochrome P450 1A2, cytochrome P450 LM4, cytochrome P450, family 1, subfamily A, polypeptide 2, cytochrome P450, family 1, subfamily a, polypeptide 2, cytochrome P450 1A2-like, CYP1A2, LOC100328937, Cyp1a2, VDBG_06455, LOC101825565, LOC102130662
    Background
    CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15 % of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.

    Synonyms: Aryl hydrocarbon hydroxylase antibody|CP 12 antibody|CP12 antibody|CP1A2_HUMAN antibody| CYP1A2 antibody|CYPIA2 antibody|Cytochrome P(3)450 antibody|Cytochrome P450 1A2 antibody| Cytochrome P450 4 antibody|Cytochrome P450 family 1 polypeptide 2 antibody|Cytochrome P450 family 1 subfamily A polypeptide 2 antibody|Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody|Cytochrome P450-P3 antibody|Cytochrome P4501A2 antibody| Dioxin inducable P3 450 antibody|Flavoprotein linked monooxygenase antibody|Microsomal monooxygenase antibody|P(3)450 antibody|P3 450 antibody|P450 4 antibody|P450 form 4 antibody|P450 P3 antibody|P450(PA) antibody|Xenobiotic monooxygenase antibody
    Gene ID
    1544
    UniProt
    P05177
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