CYP1A2 antibody (Internal Region)

Catalog No. ABIN870662

The Goat Polyclonal anti-CYP1A2 antibody is suitable to detect CYP1A2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CYP1A2 antibody (Internal Region) (ABIN870662)

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP1A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CYP1A2 Antibody

Binding Specificity: Internal Region

Purpose: Cytochrome P450 1A2 (mouse)

Sequence: HSENYKDNGG LIPEEK

Specificity: This antibody is not expected to cross-react to the similar P450 1A1.

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HSENYKDNGGLIPEEK, from the internal region of the protein sequence according to NP_034123.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Mouse fetal Liver lysates and approx. 55 kDa in Human Liver and Lung lysates (calculated MW of 58.4 kDa according to Human NP_000752.2 and 58.2 kDa according to Mouse NP_034123.1). Recommended concentration: 0.3-1

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP1A2

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Alternative Name: Cyp1a2

Background: Cyp1a2, cytochrome P450, family 1, subfamily A, polypeptide 2, CP12, Cyp1a1, P450-3, CYPIA2, aromatic compound inducible, cytochrome P450 1A2, cytochrome P450 family 1 subfamily a polypeptide 1, cytochrome P450, 1a2, aromatic compound inducible, cytochrom

Molecular Weight: 58.2kDa

Gene ID: 13077, 24297

NCBI Accession: NP_034123