FCER2 antibody (AA 50-331)

Catalog No. ABIN3043829

The Rabbit Polyclonal anti-FCER2 antibody has been validated for FACS, WB, IHC, ELISA, IF, ICC and IHC (fro). It is suitable to detect FCER2 in samples from Human, Mouse and Rat.

Quick Overview for FCER2 antibody (AA 50-331) (ABIN3043829)

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FCER2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FCER2 Antibody

Binding Specificity: AA 50-331

Purpose: Anti-CD23/FCER2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD23/FCER2 Antibody Picoband® (ABIN3043829). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA (Cap), 1-5 μg/mL, Mouse
1. Lichtman AH, Abbas AK (2003). Cellular and molecular immunology. Philadelphia: Saunders. pp. 324-325. 2. Weskamp, G., Ford, J. W., Sturgill, J., Martin, S., Docherty, A. J. P., Swendeman, S., Broadway, N., Hartmann, D., Saftig, P., Umland, S., Sehara-Fujisawa, A., Black, R. A., Ludwig, A., Becherer, J. D., Conrad, D. H., Blobel, C. P.ADAM10 is a principal 'sheddase' of the low-affinity immunoglobulin E receptor CD23.Nature Immun. 7: 1293-1298, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FCER2

Target: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Alternative Name: FCER2

Background:

Synonyms: Low affinity immunoglobulin epsilon Fc receptor,Fc-epsilon-RII,Lymphocyte IgE receptor,CD23,Fcer2,Fcer2a,

Tissue Specificity: Expressed on B-cells.

Background: CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Sequence Similarities: Contains 1 C-type lectin domain.

Molecular Weight: 49 kDa

Gene ID: 14128

UniProt: P20693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process