FCER2 antibody (AA 48-321)
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- Target See all FCER2 Antibodies
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
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Binding Specificity
- AA 48-321
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FCER2 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
- Clone
- MSVA-023M
- Isotype
- IgG
- Top Product
- Discover our top product FCER2 Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Tonsil: The follicular dendritic cells of the germinal centres should show a strong CD23 immunostaining while the majority of lymphocytes in the mantle zone of the follicles should show an at least weak staining.
Negative Control: Tonsil: The squamous epithelial cells and T-cells in the interfollicular T-zones should not show any CD23 immunostaining.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
- Alternative Name
- CD23 (FCER2 Products)
- Synonyms
- BLAST-2 antibody, CD23 antibody, CD23A antibody, CLEC4J antibody, FCE2 antibody, IGEBF antibody, Fcer2a antibody, Fc fragment of IgE receptor II antibody, FCER2 antibody, Fcer2 antibody
- Background
- BLAST-2, C-type lectin domain family 4, member J, Fc fragment of IgE low affinity II receptor, Fc-epsilon-RII, FCER2, FCER2A, FceRII, IgE-binding factor (IGEBF), Immunoglobulin E receptor, low affinity II, Lymphocyte IgE receptor,CD23 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P06734
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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