GSTA1 antibody (N-Term)

Catalog No. ABIN3043839

This anti-GSTA1 antibody is a Rabbit Polyclonal antibody detecting GSTA1 in WB, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for GSTA1 antibody (N-Term) (ABIN3043839)

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GSTA1 Antibody

Binding Specificity: AA 63-97, N-Term

Purpose: Anti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband®

Sequence: MKLVQTRAIL NYIASKYNLY GKDIKERALI DM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband® (ABIN3043839). Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GSTA1/A2/A3/A4/A5, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 2 μg/mL, Mouse, Rat
1. Atkinson, HJ, Babbitt, PC (Nov 24, 2009). "Glutathione transferases are structural and functional outliers in the thioredoxin fold.". Biochemistry 48 (46): 11108-16. 2. Boyer TD (March 1989). "The glutathione S-transferases: an update". Hepatology 9(3): 486-96.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GSTA1

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Alternative Name: GSTA1

Background:

Synonyms: Glutathione S-transferase A1, 2.5.1.18, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, Glutathione S-transferase A1, N-terminally processed, GSTA1,

Tissue Specificity: Liver.

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules). In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity, thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 26 kDa

Gene ID: 2938, 2939, 2940

UniProt: P08263