IGFBP3 antibody (C-Term)

Catalog No. ABIN3043859

There are 2+ publications for this product available. The Rabbit Polyclonal anti-IGFBP3 antibody is suitable to detect IGFBP3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for IGFBP3 antibody (C-Term) (ABIN3043859)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IGFBP3 Antibody

Binding Specificity: AA 214-252, C-Term

Purpose: Anti-IGFBP3 Antibody

Sequence: RREMEDTLNH LKFLNVLSPR GVHIPNCDKK GFYKKKQCR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IGFBP3 Antibody (ABIN3043859). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IGFBP-3, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-IGFBP3 antibody (ABIN3043859)

Pu, Wen, Liu, Zheng, Xiao, Xu, Wang, Li, Zhang: "shRNA constructs targeting IGFBP-3 alleviate age related erectile dysfunction in the rat." in: The Journal of urology, Vol. 192, Issue 3, pp. 990-6, (2014) (PubMed).

Pu, Zheng, Zhang, Xiao, Xu, Liu, Wang, Wen, Zhou, Wu: "Higher expression of mRNA and protein of insulin-like growth factor binding protein-3 in old rat penile tissues: implications for erectile dysfunction." in: The journal of sexual medicine, Vol. 8, Issue 8, pp. 2181-90, (2011) (PubMed).

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP3

Background:

Synonyms: Insulin-like growth factor-binding protein 3,IBP-3,IGF-binding protein 3,IGFBP-3,IGFBP3,IBP3,

Tissue Specificity: Expressed by most tissues. Present in plasma.

Background: IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 40 kDa

Gene ID: 3486

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding