MME antibody (AA 52-750)

Catalog No. ABIN3043879

Product Details anti-MME Antibody

Target: MME (Membrane Metallo-Endopeptidase (MME))

Binding Specificity: AA 52-750

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MME antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-CD10/MME Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD10/MME Antibody Picoband® (ABIN3043879). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Barker, P. E., Shipp, M. A., D'Adamio, L., Masteller, E. L., Reinherz, E. L. The common acute lymphoblastic leukemia antigen gene maps to chromosomal region 3(q21-q27). J. Immun. 142: 283-287, 1989. 2. Northridge, D. B., Jardine, A. G., Alabaster, C. T., Barclay, P. L., Connell, J. M. C., Dargie, H. J., Dilly, S. G., Findlay, I. N., Lever, A. F., Samuels, G. M. R. Effects of UK 69 578: a novel atriopeptidase inhibitor. Lancet 332: 591-593, 1989. Note: Originally Volume II. 3. Stephenson, S. L., Kenny, A. J. The hydrolysis of human atrial natriuretic peptide by pig kidney microvillar membranes is initiated by endopeptidase-24.11. Biochem. J. 243: 183-187, 1987. 4 Tran-Paterson, R., Willard, H. F., Letarte, M. The common acute lymphoblastic leukemia antigen (neutral endopeptidase--3.4.24.11) gene is located on human chromosome 3. Cancer Genet. Cytogenet. 42: 129-134, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-MME antibody (ABIN3043879)

Wang, Tian, Xiao, Zhang, Sun, Tang: "A metanephric adenoma of the kidney associated with polycythemia: A case report." in: Oncology letters, Vol. 11, Issue 1, pp. 352-354, (2016) (PubMed).

Meng, Li, Geng, Ohe, Yu, E, Ye, Yang, Kato, Zhang, Ishida, Ohta, Jin, Kakehata, Geng, Yamakawa: "Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy." in: Translational research : the journal of laboratory and clinical medicine, Vol. 166, Issue 3, pp. 281-91, (2015) (PubMed).

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: MME (MME Products)

Background:

Synonyms: Neprilysin,3.4.24.11 ,Atriopeptidase,Common acute lymphocytic leukemia antigen,CALLA,Enkephalinase,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Skin fibroblast elastase,SFE,CD10,MME,EPN,

Tissue Specificity: Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, or common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Sequence Similarities: Belongs to the peptidase M13 family.

Molecular Weight: 100 kDa

Gene ID: 4311

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration