MMP8 antibody (N-Term)

Catalog No. ABIN3043882

This Rabbit Polyclonal antibody specifically detects MMP8 in WB, IHC and ELISA. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for MMP8 antibody (N-Term) (ABIN3043882)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MMP8 Antibody

Binding Specificity: AA 119-153, N-Term

Purpose: Anti-MMP8 Antibody Picoband®

Sequence: NYTPQLSEAE VERAIKDAFE LWSVASPLIF TRISQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MMP8 Antibody Picoband® (ABIN3043882). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MMP-8, different from the related mouse sequence by eleven amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human
1. Balbin, M., Fueyo, A., Tester, A. M., Pendas, A. M., Pitiot, A. S., Astudillo, A., Overall, C. M., Shapiro, S. D., Lopez-Otin, C. : Loss of collagenase-2 confers increased skin tumor susceptibility to male mice. Nature Genet. 35: 252-257, 2003. 2. Devarajan, P., Mookhtiar, K., Van Wart, H., Berliner, N. : Structure and expression of the cDNA encoding human neutrophil collagenase. Blood 77: 2731-2738, 1991. 3. Hasty, K. A., Pourmotabbed, T. F., Goldberg, G. I., Thompson, J. P., Spinella, D. G., Stevens, R. M., Mainardi, C. L. : Human neutrophil collagenase: a distinct gene product with homology to other matrix metalloproteinases. J. Biol. Chem. 265: 11421-11424, 1990. 4. Wang, H., Parry, S., Macones, G., Sammel, M. D., Ferrand, P. E., Kuivaniemi, H., Tromp, G., Halder, I., Shriver, M. D., Romero, R., Strauss, J. F., III : Functionally significant SNP MMP8 promoter haplotypes and preterm premature rupture of membranes (PPROM). Hum. Molec. Genet. 13: 2659-2669, 2004.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-MMP8 antibody (ABIN3043882)

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Li, Li, Zeng, Liu, Yang: "H2S improves renal fibrosis in STZ-induced diabetic rats by ameliorating TGF-β1 expression." in: Renal failure, Vol. 39, Issue 1, pp. 265-272, (2017) (PubMed).

Gao, Tang, He, Liu, Mao, Ji, Lin, Wu: "Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats." in: Frontiers in pharmacology, Vol. 6, pp. 215, (2015) (PubMed).

Zhong, Wang, Jian: "Expression of matrix metalloproteinases-8 and -9 and their tissue inhibitor in the condyles of diabetic rats with mandibular advancement." in: Experimental and therapeutic medicine, Vol. 8, Issue 5, pp. 1357-1364, (2014) (PubMed).

Target Details for MMP8

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP8

Background:

Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

Tissue Specificity: Neutrophils.

Background: MMP8 (Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases. It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8 is mapped to 11q21-q22. MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The enzyme exhibits 58 % homology to human fibroblast collagenase and has the same domain structure. It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond. MMP8 was found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Matrix metalloproteinases (MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer. MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility.

Molecular Weight: 60 kDa

Gene ID: 4317

UniProt: P22894