Parkin antibody (AA 23-416)

Catalog No. ABIN3043896

Product Details anti-Parkin Antibody

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Binding Specificity: AA 23-416

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Parkin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase parkin(PARK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase parkin(PARK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: parkin RBR E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase parkin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Parkin is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: PARK2 (PARK2 Products)

Synonyms: CG10523 antibody, Dmel\\CG10523 antibody, Dpark antibody, SD01679 antibody, dpk antibody, AR-JP antibody, LPRS2 antibody, PDJ antibody, PRKN antibody, Park antibody, Prkn antibody, si:ch211-123f21.1 antibody, zgc:112390 antibody, pdr-1 antibody, PARK2 antibody, parkin antibody, parkin RBR E3 ubiquitin protein ligase antibody, Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody, park antibody, PRKN antibody, Prkn antibody, prkn antibody, CpipJ_CPIJ014867 antibody, Park2 antibody

Background: Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.

Synonyms: AR JP antibody|E3 ubiquitin ligase antibody|E3 ubiquitin protein ligase parkin antibody|E3 ubiquitin-protein ligase parkin antibody|FRA6E antibody|LPRS 2 antibody|LPRS2 antibody|PARK 2 antibody|PARK2 antibody|Parkin 2 antibody|Parkinson disease (autosomal recessive juvenile) 2 antibody|Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody|Parkinson disease protein 2 antibody|Parkinson juvenile disease protein 2 antibody|Parkinson protein 2 E3 ubiquitin protein ligase antibody|Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody|PDJ antibody|PRKN 2 antibody|PRKN antibody|PRKN2 antibody|PRKN2_HUMAN antibody|Ubiquitin E3 ligase PRKN antibody

Gene ID: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway